Precision cancer mouse models through genome editing with CRISPR-Cas9
Name
13073_2015_Article_178.pdf
Size
1.61 MB
Format
Adobe PDF
Checksum (MD5)
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Author(s)
Mou, Haiwei
Kennedy, Zachary
Yin, Hao
Xue, Wen
Anderson, Daniel Griffith
Date Issued
June 2015
Journal
Genome Medicine
Publisher
BioMed Central
Citation
Mou, Haiwei, Zachary Kennedy, Daniel G. Anderson, Hao Yin, and Wen Xue. “Precision Cancer Mouse Models through Genome Editing with CRISPR-Cas9.” Genome Medicine 7, no. 1 (June 9, 2015).
Version
Final published version
Abstract
The cancer genome is highly complex, with hundreds of point mutations, translocations, and chromosome gains and losses per tumor. To understand the effects of these alterations, precise models are needed. Traditional approaches to the construction of mouse models are time-consuming and laborious, requiring manipulation of embryonic stem cells and multiple steps. The recent development of the clustered regularly interspersed short palindromic repeats (CRISPR)-Cas9 system, a powerful genome-editing tool for efficient and precise genome engineering in cultured mammalian cells and animals, is transforming mouse-model generation. Here, we review how CRISPR-Cas9 has been used to create germline and somatic mouse models with point mutations, deletions and complex chromosomal rearrangements. We highlight the progress and challenges of such approaches, and how these models can be used to understand the evolution and progression of individual tumors and identify new strategies for cancer treatment. The generation of precision cancer mouse models through genome editing will provide a rapid avenue for functional cancer genomics and pave the way for precision cancer medicine.
MIT Department
Massachusetts Institute of Technology. Institute for Medical Engineering & Science
Harvard University--MIT Division of Health Sciences and Technology
Massachusetts Institute of Technology. Department of Chemical Engineering
Koch Institute for Integrative Cancer Research at MIT
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1186/s13073-015-0178-7
