Comparison of cellular responses induced by low level light in different cell types
Name
Huang-2010-Comparison of cellular.pdf
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Author(s)
Hamblin, Michael R.
Huang, Ying-Ying
Chen, Aaron Chih-Hao
Sharma, Sulbha K.
Wu, Qiuhe
Date Issued
February 2010
Journal
Proceedings of SPIE--the International Society for Optical Engineering
Publisher
Society of Photo-optical Instrumentation Engineers
Citation
Huang, Ying-Ying et al. “Comparison of cellular responses induced by low level light in different cell types.” Mechanisms for Low-Light Therapy V. Ed. Michael R. Hamblin, Ronald W. Waynant, & Juanita Anders. San Francisco, California, USA: SPIE, 2010. 75520A-10. ©2010 SPIE--The International Society for Optical Engineering.
Version
Final published version
Abstract
Discoveries are rapidly being made in multiple laboratories that shed "light" on the fundamental molecular and cellular mechanisms underlying the use of low level light therapy (LLLT) in vitro, in animal models and in clinical practice. Increases in cellular levels of respiration, in cytochrome c oxidase activity, in ATP levels and in cyclic AMP have been found. Increased expression of reactive oxygen species and release of nitric oxide have also been shown. In order for these molecular changes to have a major effect on cell behavior, it is likely that various transcription factors will be activated, possibly via different signal transduction pathways. In this report we compare and contrast the effects of LLLT in vitro on murine embryonic fibroblasts, primary cortical neurons, cardiomyocytes and bone-marrow derived dendritic cells. We also examined two human cell lines, HeLa cancer cells and HaCaT keratinocytes. The effects of 810-nm near-infra-red light delivered at low and high fluences were addressed. Reactive oxygen species generation, transcription factor activation and ATP increases are reported. The data has led to the hypothesis that cells with a high level of mitochondrial activity (mitochondrial membrane potential) have a higher response to light than cells with low mitochondrial activity.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
http://dx.doi.org/10.1117/12.841018
