Peptide targeting and imaging of damaged lung tissue in influenza-infected mice
Name
Engelward_Peptide targeting paper and SI.pdf
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1.97 MB
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Author(s)
Li, Na
Yin, Lu
Thevenin, Damien
Yamada, Yoshiyuki
Limmon, Gino V.
Chen, Jianzhu
Chow, Vincent T. K.
Engelman, Donald M.
Engelward, Bevin P.
Date Issued
February 2013
Journal
Future Microbiology
Publisher
Future Medicine
Citation
Li, Na et al. “Peptide Targeting and Imaging of Damaged Lung Tissue in Influenza-infected Mice.” Future Microbiology 8.2 (2013): 257–269. CrossRef. Web.
Version
Author's final manuscript
Abstract
In this study, we investigate whether pH (low) insertion peptide (pHLIP) can target regions of lung injury associated with influenza infection. Materials & methods: Fluorophore-conjugated pHLIP was injected intraperitoneally into mice infected with a sublethal dose of H1N1 influenza and visualized histologically. Results: pHLIP specifically targeted inflamed lung tissues of infected mice in the later stages of disease and at sites where alveolar type I and type II cells were depleted. Regions of pHLIP-targeted lung tissue were devoid of peroxiredoxin 6, the lung-abundant antioxidant enzyme, and were deficient in pneumocytes. Interestingly, a pHLIP variant possessing mutations that render it insensitive to pH changes was also able to target damaged lung tissue. Conclusion: pHLIP holds potential for delivering therapeutics for lung injury during influenza infection. Furthermore, there may be more than one mechanism that enables pHLIP variants to target inflamed lung tissue.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Biology
Singapore-MIT Alliance in Research and Technology (SMART)
Koch Institute for Integrative Cancer Research at MIT
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike 3.0
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DOI of Published Version
https://doi.org/10.2217/fmb.12.134
