Single-Shot Quantitative Polarization Imaging of Complex Birefringent Structure Dynamics
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2106.06118v1.pdf
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Author(s)
Ge, Baoliang
Zhang, Qing
Zhang, Rui
Lin, Jing-Tang
Tseng, Po-Hang
Chang, Che-Wei
Dong, Chen-Yuan
Zhou, Renjie
Yaqoob, Zahid
Bischofberger, Irmgard
Date Issued
November 17, 2021
Journal
ACS Photonics
Publisher
American Chemical Society (ACS)
Citation
Ge, Baoliang, Zhang, Qing, Zhang, Rui, Lin, Jing-Tang, Tseng, Po-Hang et al. 2021. "Single-Shot Quantitative Polarization Imaging of Complex Birefringent Structure Dynamics." ACS Photonics, 8 (12).
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Author's final manuscript
Abstract
Polarization light microscopes are powerful tools for probing molecular order and orientation in birefringent materials. While a number of polarization microscopy techniques are available to access steady-state properties of birefringent samples, quantitative measurements of the molecular orientation dynamics on the millisecond time scale have remained a challenge. We propose polarized shearing interference microscopy (PSIM), a single-shot quantitative polarization imaging method, for extracting the retardance and orientation angle of the laser beam transmitting through optically anisotropic specimens with complex structures. The measurement accuracy and imaging performance of PSIM are validated by imaging a birefringent resolution target and a bovine tendon specimen. We demonstrate that PSIM can quantify the dynamics of a flowing lyotropic chromonic liquid crystal in a microfluidic channel at an imaging speed of 506 frames per second (only limited by the camera frame rate), with a field-of-view of up to 350 × 350 μm2 and a diffraction-limit spatial resolution of ~2 μm. We envision that PSIM will find a broad range of applications in quantitative material characterization under dynamical conditions.
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
Massachusetts Institute of Technology. Laser Biomedical Research Center
Massachusetts Institute of Technology. Department of Biological Engineering
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DOI of Published Version
https://doi.org/10.1021/acsphotonics.1c00788
