DNA Directed Assembly Probe for Detecting DNA-Protein Interaction in Microarray Format
Author(s)
Ng, Jin Kiat
Ajikumar, Parayil Kumaran
Tang, Yew Chung
Stephanopoulos, Gregory
Too, Heng-Phon
Date Issued
January 2007
Series/Report no.
Chemical and Pharmaceutical Engineering (CPE)
Abstract
Quantifying DNA-protein interaction using DNA microarrays are gaining increasing attention due to their ability to profile specificity of interactions in a high-throughput manner. This paper describes a new approach that used the
ability of ssDNA-dsDNA probe to complex with DNA binding proteins in the solution phase and then spatially immobilized onto microarray through specific DNA hybridization. In one case, the Spatially Addressable DNA Array
(SADA) approach demonstrated that
enzymatic cleavage in solution is more
efficient than if conducted heterogeneously. In addition, binding of RNA polymerase with promoter DNA could be detected with this strategy.
Subjects
DNA-Protein Interaction
DNA Directed Assembly
Restriction Enzyme
RNA Polymerase
ssDNA-dsDNA Conjugate
Persistent DSpace Link
