High‐Throughput Microfluidic‐Mediated Assembly of Layer‐By‐Layer Nanoparticles
Name
Adv Funct Materials - 2025 - Pires - High‐Throughput Microfluidic‐Mediated Assembly of Layer‐By‐Layer Nanoparticles.pdf
Description
Published version
Size
3.03 MB
Format
Adobe PDF
Checksum (MD5)
f2994e86b62bdac93d88890cf132dc59
Author(s)
Pires, Ivan S
Gordon, Ezra
Suh, Heikyung
Irvine, Darrell J
Hammond, Paula T
Date Issued
April 3, 2025
Journal
Advanced Functional Materials
Publisher
Wiley
Citation
Pires, Ivan S, Gordon, Ezra, Suh, Heikyung, Irvine, Darrell J and Hammond, Paula T. 2025. "High‐Throughput Microfluidic‐Mediated Assembly of Layer‐By‐Layer Nanoparticles." Advanced Functional Materials.
Version
Final published version
Abstract
Surface modification of nanoparticles (NPs) via the layer‐by‐layer (LbL) technique is a promising approach to generate targeted drug delivery vehicles. LbL‐NPs have been successfully used in preclinical models for controlled drug release, tumor and immune cell targeting, improved pharmacokinetics and biodistribution, and controlling cellular trafficking and uptake mechanisms. A simple and scalable synthesis method for LbL‐NPs that can be adapted for clinical translation is of great interest. Here a new method of polymer deposition is presented onto NPs enabled through microfluidic (MCF) mixing. NPs are mixed with polyelectrolytes using commercially available bifurcating mixer MCF cartridges. In addition to increased process robustness, MCF allows for LbL electrostatic assembly using titrated polymer‐to‐NP weight equivalent ratios where no excess polymer is required to achieve a given LbL layering. Under such conditions, no time‐consuming purification is needed, greatly increasing LbL‐NP throughput and avoiding the loss of NPs during purification. The utility of this system is demonstrated using interleukin‐12‐loaded liposomal NPs, which show equivalent efficacy in vitro and in vivo to LbL‐NPs generated via traditional lab‐scale batch‐wise polymer adsorption and tangential flow filtration purification. Moreover, it is shown that MCF can assemble LbL films of various chemistries and on various NP core substrates.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Materials Science and Engineering
Koch Institute for Integrative Cancer Research at MIT
Massachusetts Institute of Technology. Department of Chemical Engineering
Ragon Institute of MGH, MIT and Harvard
Ragon Institute of MGH, MIT and Harvard
Ragon Institute of MGH, MIT and Harvard
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Creative Commons Attribution
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DOI of Published Version
https://doi.org/10.1002/adfm.202503965
