Genome-wide Maps of Nuclear Lamina Interactions in Single Human Cells
Name
nihms718969.pdf
Size
2.2 MB
Format
Adobe PDF
Checksum (MD5)
f14e3cdba6a99eedb33312b8694d32b5
Author(s)
Kind, Jop
Pagie, Ludo
de Vries, Sandra S.
Nahidiazar, Leila
Dey, Siddharth S.
Bienko, Magda
Zhan, Ye
Lajoie, Bryan
de Graaf, Carolyn A.
Amendola, Mario
Date Issued
September 2015
Journal
Cell
Publisher
Elsevier
Citation
Kind, Jop et al. “Genome-Wide Maps of Nuclear Lamina Interactions in Single Human Cells.” Cell 163, 1 (September 2015): 134–147 © 2015 Elsevier Inc
Version
Author's final manuscript
Abstract
Mammalian interphase chromosomes interact with the nuclear lamina (NL) through hundreds of large lamina-associated domains (LADs). We report a method to map NL contacts genome-wide in single human cells. Analysis of nearly 400 maps reveals a core architecture consisting of gene-poor LADs that contact the NL with high cell-to-cell consistency, interspersed by LADs with more variable NL interactions. The variable contacts tend to be cell-type specific and are more sensitive to changes in genome ploidy than the consistent contacts. Single-cell maps indicate that NL contacts involve multivalent interactions over hundreds of kilobases. Moreover, we observe extensive intra-chromosomal coordination of NL contacts, even over tens of megabases. Such coordinated loci exhibit preferential interactions as detected by Hi-C. Finally, the consistency of NL contacts is inversely linked to gene activity in single cells and correlates positively with the heterochromatic histone modification H3K9me3. These results highlight fundamental principles of single-cell chromatin organization.
MIT Department
Massachusetts Institute of Technology. Department of Physics
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1016/J.CELL.2015.08.040
