RNAi screen reveals a role for PACSIN2 and caveolins during bacterial cell-to-cell spread
Name
mbc.e19-04-0197.pdf
Description
Published version
Size
3.32 MB
Format
Adobe PDF
Checksum (MD5)
acd0930288d0e328687b3c41a9dc5707
Author(s)
Sanderlin, Allen G
Vondrak, Cassandra
Scricco, Arianna J.
Scricco, Arianna J.
Fedrigo, Indro
Ahyong, Vida
Lamason, Rebecca L.
Date Issued
August 2019
Journal
Molecular Biology of the Cell
Publisher
American Society for Cell Biology (ASCB)
Citation
Sanderlin, Allen G. et al. "RNAi screen reveals a role for PACSIN2 and caveolins during bacterial cell-to-cell spread." Molecular Biology of the Cell 30, 17 (August 2019): 2097-2347 © 2019 The Author(s)
Version
Final published version
Abstract
Listeria monocytogenes is a human bacterial pathogen that disseminates through host tissues using a process called cell-to-cell spread. This critical yet understudied virulence strategy resembles a vesicular form of intercellular trafficking that allows L. monocytogenes to move between host cells without escaping the cell. Interestingly, eukaryotic cells can also directly exchange cellular components via intercellular communication pathways (e.g., trans-endocytosis) using cell–cell adhesion, membrane trafficking, and membrane remodeling proteins. Therefore, we hypothesized that L. monocytogenes would hijack these types of host proteins during spread. Using a focused RNA interference screen, we identified 22 host genes that are important for L. monocytogenes spread. We then found that caveolins (CAV1 and CAV2) and the membrane sculpting F-BAR protein PACSIN2 promote L. monocytogenes protrusion engulfment during spread, and that PACSIN2 specifically localizes to protrusions. Overall, our study demonstrates that host intercellular communication pathways may be coopted during bacterial spread and that specific trafficking and membrane remodeling proteins promote bacterial protrusion resolution.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Creative Commons Attribution Noncommercial 3.0 unported license
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DOI of Published Version
https://doi.org/10.1091/mbc.e19-04-0197
