Combinatorial Library of Lipidoids for In Vitro DNA Delivery
Name
Langer_Combinatorial library.pdf
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Author(s)
Sun, Shuo
Wang, Ming
Knupp, Sarah A.
Hu, Xiao
Kaplan, David L.
Xu, Qiaobing
Soto Feliciano, Yadira Marie
Langer, Robert S
Anderson, Daniel Griffith
Date Issued
January 2012
Journal
Bioconjugate Chemistry
Publisher
American Chemical Society
Citation
Sun, Shuo, Ming Wang, Sarah A. Knupp, Yadira Soto-Feliciano, Xiao Hu, David L. Kaplan, Robert Langer, Daniel G. Anderson, and Qiaobing Xu. Combinatorial Library of Lipidoids for In Vitro DNA Delivery. Bioconjugate Chemistry 23, no. 1 (January 18, 2012): 135-140.
Version
Author's final manuscript
Abstract
A combinatorial library of lipidoids was constructed and studied for in vitro gene delivery. The library of lipidoids was synthesized by reacting commercially available amines with lipophilic acrylates, acrylamides, or epoxides. Lipidoids derived from amine 86 (N,N-bis(2-hydroxyethyl)ethylene diamine) and amine 87 (N-(3-aminopropyl)diethaneamine) showed high efficiency in DNA delivery, some with a higher transfection efficiency than Lipofectamine 2000, a commonly used commercial gold standard for in vitro gene delivery. The structure–activity relationship between the lipidoids was further studied with respect to small variations in chemical structures and the resulting efficiency in DNA delivery in vitro. Since these lipidoids are easy to synthesize and do not require a colipid for efficient DNA delivery, they could offer an inexpensive but effective alternative to other commonly used commercial gene delivery carriers.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Koch Institute for Integrative Cancer Research at MIT
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1021/bc200572w
