3' UTR-isoform choice has limited influence on the stability and translational efficiency of most mRNAs in mouse fibroblasts
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Spies-2013-3 ' UTR-isoform choi.pdf
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1.6 MB
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Author(s)
Spies, Noah
Bartel, David
Burge, Christopher B
Date Issued
September 2013
Journal
Genome Research
Publisher
Cold Spring Harbor Laboratory Press
Citation
Spies, N., C. B. Burge, and D. P. Bartel. “3’ UTR-Isoform Choice Has Limited Influence on the Stability and Translational Efficiency of Most mRNAs in Mouse Fibroblasts.” Genome Research 23, no. 12 (December 1, 2013): 2078–2090.
Version
Final published version
Abstract
Variation in protein output across the genome is controlled at several levels, but the relative contributions of different regulatory mechanisms remain poorly understood. Here, we obtained global measurements of decay and translation rates for mRNAs with alternative 3′ untranslated regions (3′ UTRs) in murine 3T3 cells. Distal tandem isoforms had slightly but significantly lower mRNA stability and greater translational efficiency than proximal isoforms on average. The diversity of alternative 3′ UTRs also enabled inference and evaluation of both positively and negatively acting cis-regulatory elements. The 3′ UTR elements with the greatest implied influence were microRNA complementary sites, which were associated with repression of 32% and 4% at the stability and translational levels, respectively. Nonetheless, both the decay and translation rates were highly correlated for proximal and distal 3′ UTR isoforms from the same genes, implying that in 3T3 cells, alternative 3′ UTR sequences play a surprisingly small regulatory role compared to other mRNA regions.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Biology
Whitehead Institute for Biomedical Research
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Creative Commons Attribution-Noncommerical
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DOI of Published Version
https://doi.org/10.1101/gr.156919.113
