Resonant microchannel volume and mass measurements show that suspended cells swell during mitosis
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Son-2015-Resonant microchanne.pdf
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Author(s)
Son, Sungmin
Kang, Joon Ho
Oh, Seungeun
Kirschner, Marc W.
Mitchison, T.J.
Manalis, Scott R
Date Issued
November 2015
Journal
Journal of Cell Biology
Publisher
Rockefeller University Press
Citation
Son, Sungmin, Joon Ho Kang, Seungeun Oh, Marc W. Kirschner, T.J. Mitchison, and Scott Manalis. “Resonant Microchannel Volume and Mass Measurements Show That Suspended Cells Swell During Mitosis.” Journal of Cell Biology 211, no. 4 (November 23, 2015): 757–763.
Version
Final published version
Abstract
Osmotic regulation of intracellular water during mitosis is poorly understood because methods for monitoring relevant cellular physical properties with sufficient precision have been limited. Here we use a suspended microchannel resonator to monitor the volume and density of single cells in suspension with a precision of 1% and 0.03%, respectively. We find that for transformed murine lymphocytic leukemia and mouse pro–B cell lymphoid cell lines, mitotic cells reversibly increase their volume by more than 10% and decrease their density by 0.4% over a 20-min period. This response is correlated with the mitotic cell cycle but is not coupled to nuclear osmolytes released by nuclear envelope breakdown, chromatin condensation, or cytokinesis and does not result from endocytosis of the surrounding fluid. Inhibiting Na-H exchange eliminates the response. Although mitotic rounding of adherent cells is necessary for proper cell division, our observations that suspended cells undergo reversible swelling during mitosis suggest that regulation of intracellular water may be a more general component of mitosis than previously appreciated.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Mechanical Engineering
Massachusetts Institute of Technology. Department of Physics
Koch Institute for Integrative Cancer Research at MIT
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DOI of Published Version
https://doi.org/10.1083/jcb.201505058
