High spatial and temporal resolution synthetic aperture phase microscopy
Name
065002_1.pdf
Description
Published version
Size
3.97 MB
Format
Adobe PDF
Checksum (MD5)
df4f22eda7133453b47fd2d7fe447fae
Author(s)
Zheng, Cheng
Jin, Di
He, Yanping
Lin, Hongtao
Hu, Juejun
Yaqoob, Zahid
So, Peter TC
Zhou, Renjie
Date Issued
2020
Journal
Advanced Photonics
Publisher
SPIE-Intl Soc Optical Eng
Citation
Zheng, Cheng, Jin, Di, He, Yanping, Lin, Hongtao, Hu, Juejun et al. 2020. "High spatial and temporal resolution synthetic aperture phase microscopy." Advanced Photonics, 2 (06).
Version
Final published version
Abstract
A new optical microscopy technique, termed high spatial and temporal resolution synthetic aperture phase microscopy (HISTR-SAPM), is proposed to improve the lateral resolution of wide-field coherent imaging. Under plane wave illumination, the resolution is increased by twofold to around 260 nm, while achieving millisecond-level temporal resolution. In HISTR-SAPM, digital micromirror devices are used to actively change the sample illumination beam angle at high speed with high stability. An off-axis interferometer is used to measure the sample scattered complex fields, which are then processed to reconstruct high-resolution phase images. Using HISTR-SAPM, we are able to map the height profiles of subwavelength photonic structures and resolve the period structures that have 198 nm linewidth and 132 nm gap (i.e., a full pitch of 330 nm). As the reconstruction averages out laser speckle noise while maintaining high temporal resolution, HISTR-SAPM further enables imaging and quantification of nanoscale dynamics of live cells, such as red blood cell membrane fluctuations and subcellular structure dynamics within nucleated cells. We envision that HISTR-SAPM will broadly benefit research in material science and biology.
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
Massachusetts Institute of Technology. Department of Materials Science and Engineering
Massachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory
Massachusetts Institute of Technology. Laser Biomedical Research Center
Massachusetts Institute of Technology. Department of Biological Engineering
Terms of Use
Creative Commons Attribution 4.0 International license
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1117/1.AP.2.6.065002
