The E2 Ubiquitin-conjugating Enzyme UBE2J1 Is Required for Spermiogenesis in Mice
Name
Koenig_Manuscript_JBiolChem_revised.pdf
Size
3.53 MB
Format
Adobe PDF
Checksum (MD5)
5f366a8e2f5dbda3ec53af173a64027c
Author(s)
Koenig, Paul-Albert
Hagiwara, Masatoshi
Maruyama, Takeshi
Watson, Nicki
Page, David C
Nicholls, Peter
Schmidt, Florian Ingo
Ploegh, Hidde
Frydman, Galit H.
Date Issued
October 2014
Journal
Journal of Biological Chemistry
Publisher
American Society for Biochemistry and Molecular Biology (ASBMB)
Citation
Koenig, Paul-Albert, Peter K. Nicholls, Florian I. Schmidt, Masatoshi Hagiwara, Takeshi Maruyama, Galit H. Frydman, Nicki Watson, David C. Page, and Hidde L. Ploegh. “The E2 Ubiquitin-Conjugating Enzyme UBE2J1 Is Required for Spermiogenesis in Mice.” Journal of Biological Chemistry 289, no. 50 (October 15, 2014): 34490–34502. © 2014 American Society for Biochemistry and Molecular Biology, Inc. (ASBMB)
Version
Author's final manuscript
Abstract
ER-resident proteins destined for degradation are dislocated into the cytosol by components of the ER quality control machinery for proteasomal degradation. Dislocation substrates are ubiquitylated in the cytosol by E2 ubiquitin-conjugating/E3 ligase complexes. UBE2J1 is one of the well-characterized E2 enzymes that participate in this process. However, the physiological function of Ube2j1 is poorly defined. We find that Ube2j1−/− mice have reduced viability and fail to thrive early after birth. Male Ube2j1−/− mice are sterile due to a defect in late spermatogenesis. Ultrastructural analysis shows that removal of the cytoplasm is incomplete in Ube2j1−/− elongating spermatids, compromising the release of mature elongate spermatids into the lumen of the seminiferous tubule. Our findings identify an essential function for the ubiquitin-proteasome-system in spermiogenesis and define a novel, non-redundant physiological function for the dislocation step of ER quality control.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Massachusetts Institute of Technology. Division of Comparative Medicine
Whitehead Institute for Biomedical Research
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1074/jbc.M114.604132
