The etiology of uracil residues in the Saccharomyces cerevisiae genomic DNA
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294_2018_Article_895.pdf
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Author(s)
Stokdyk, Kasey
Kim, Nayun
Owiti, Norah Auma
Date Issued
October 2018
Journal
Current Genetics
Publisher
Springer Berlin Heidelberg
Citation
Owiti, Norah et al. “The Etiology of Uracil Residues in the Saccharomyces Cerevisiae Genomic DNA.” Current Genetics (October 2018): 1-7 © 2018 The Author(s)
Version
Final published version
Abstract
Non-canonical residue in DNA is a major and conserved source of genome instability. The appearance of uracil residues in DNA accompanies a significant mutagenic consequence and is regulated at multiple levels, from the concentration of available dUTP in the nucleotide pool to the excision repair for removal from DNA. Recently, an interesting phenomenon of transcription-associated elevation in uracil-derived mutations was described in Saccharomyces cerevisiae genome. While trying to understand the variability in mutagenesis, we uncovered that the frequency of uracil incorporation into DNA can vary depending on the transcription rate and that the non-replicative, repair-associated DNA synthesis underlies the higher uracil density of the actively transcribed genomic loci. This novel mechanism brings together the chemical vulnerability of DNA under transcription and the uracil-associated mutagenesis, and has the potential to apply to other non-canonical residues of mutagenic importance. Keyword: DNA repair; Non-canonical nucleotides; Transcription-associated mutagenesis; Uracil dUTPase
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
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Creative Commons Attribution
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DOI of Published Version
https://doi.org/10.1007/s00294-018-0895-8
