Regulatory Cohesion of Cell Cycle and Cell Differentiation through Interlinked Phosphorylation and Second Messenger Networks
Name
Baker_Regulatory cohesion.pdf
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1.29 MB
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Author(s)
Abel, Sören
Chien, Peter
Wassmann, Paul
Schirmer, Tilman
Kaever, Volkhard
Baker, Tania
Jenal, Urs
Laub, Michael T.
Laub, Michael T
Date Issued
August 2011
Journal
Molecular Cell
Publisher
Elsevier B.V.
Citation
Abel, Soren, Peter Chien, Paul Wassmann, Tilman Schirmer, Volkhard Kaever, Michael T. Laub, Tania A. Baker, and Urs Jenal. “Regulatory Cohesion of Cell Cycle and Cell Differentiation through Interlinked Phosphorylation and Second Messenger Networks.” Molecular Cell 43, no. 4 (August 2011): 550-560. © 2011 Elsevier Inc.
Version
Final published version
Abstract
In Caulobacter crescentus, phosphorylation of key regulators is coordinated with the second messenger cyclic di-GMP to drive cell-cycle progression and differentiation. The diguanylate cyclase PleD directs pole morphogenesis, while the c-di-GMP effector PopA initiates degradation of the replication inhibitor CtrA by the AAA+ protease ClpXP to license S phase entry. Here, we establish a direct link between PleD and PopA reliant on the phosphodiesterase PdeA and the diguanylate cyclase DgcB. PdeA antagonizes DgcB activity until the G1-S transition, when PdeA is degraded by the ClpXP protease. The unopposed DgcB activity, together with PleD activation, upshifts c-di-GMP to drive PopA-dependent CtrA degradation and S phase entry. PdeA degradation requires CpdR, a response regulator that delivers PdeA to the ClpXP protease in a phosphorylation-dependent manner. Thus, CpdR serves as a crucial link between phosphorylation pathways and c-di-GMP metabolism to mediate protein degradation events that irreversibly and coordinately drive bacterial cell-cycle progression and development.
MIT Department
Massachusetts Institute of Technology. Department of Biology
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DOI of Published Version
https://doi.org/10.1016/j.molcel.2011.07.018
