Engineered autonomous dynamic regulation of metabolic flux
Name
Nature Review_Clean_with figures.pdf
Description
Accepted version
Size
626.67 KB
Format
Adobe PDF
Checksum (MD5)
138670d2ee3d2c46cdd35a2e563f5c14
Author(s)
Ream, Michael
Prather, Kristala LJ
Date Issued
December 21, 2023
Journal
Nature Reviews Bioengineering
Publisher
Springer Science and Business Media LLC
Citation
Ream, M., Prather, K.L.J. Engineered autonomous dynamic regulation of metabolic flux. Nat Rev Bioeng 2, 233–243 (2024).
Version
Author's final manuscript
Abstract
Metabolic engineering is a powerful tool to reprogramme cells to produce value-added chemicals. Such engineering strategies require the fine-tuning of a cell’s metabolism to balance competition for resources and prevent negative impacts on growth. Dynamic regulation enables the shifting of resources or metabolic flux toward different pathways based on a received input to increase titres of value-added chemicals in microbial production strains. In this Review, we discuss autonomous dynamic regulation, that is, responses triggered directly by a stimulus without the need for human intervention, and its application to metabolic engineering. We highlight strategies to control the transcription of genes using metabolite-specific regulation, including by transcription factors and through biosensing, and non-specific regulation, in particular, environmental regulation, growth-phase responses and quorum sensing, examining the application of these regulation strategies to the bioproduction of different chemicals.
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1038/s44222-023-00140-7
