Recent advances in chromosome capture techniques unraveling 3D genome architecture in germ cells, health, and disease
Name
10142_2023_1146_ReferencePDF.pdf
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3.08 MB
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Checksum (MD5)
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Author(s)
Pandupuspitasari, Nuruliarizki S.
Khan, Faheem A.
Huang, Chunjie
Ali, Azhar
Yousaf, Muhammad R.
Shakeel, Farwa
Putri, Ezi M.
Negara, Windu
Muktiani, Anis
Prasetiyono, Bambang W. H. E.
Date Issued
June 29, 2023
Publisher
Springer Berlin Heidelberg
Citation
Functional & Integrative Genomics. 2023 Jun 29;23(3):214
Version
Author's final manuscript
Abstract
Abstract
In
eukaryotes, the genome
does not emerge in a specific shape but rather as a hierarchial bundle within the nucleus. This multifaceted genome organization consists of multiresolution cellular structures, such as chromosome territories, compartments, and topologically associating domains, which are frequently defined by architecture, design proteins including CTCF and cohesin, and chromatin loops. This review briefly discusses the advances in understanding the basic rules of control, chromatin folding, and functional areas in early embryogenesis. With the use of chromosome capture techniques, the latest advancements in technologies for visualizing chromatin interactions come close to revealing 3D genome formation frameworks with incredible detail throughout all genomic levels, including at single-cell resolution. The possibility of detecting variations in chromatin architecture might open up new opportunities for disease diagnosis and prevention, infertility treatments, therapeutic approaches, desired exploration, and many other application scenarios.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1007/s10142-023-01146-5
