Three-Dimensional Characterization of Mechanical Interactions between Endothelial Cells and Extracellular Matrix during Angiogenic Sprouting
Name
Du-2016-Three-dimensional.pdf
Size
2.61 MB
Format
Adobe PDF
Checksum (MD5)
2a165b623c37514723f65b54e1531fc9
Author(s)
Du, Yue
Herath, Sahan C. B.
Wang, Qing-guo
Wang, Dong-an
Chen, Peter C. Y.
Asada, Haruhiko
Date Issued
February 2016
Journal
Scientific Reports
Publisher
Nature Publishing Group
Citation
Du, Yue, Sahan C. B. Herath, Qing-guo Wang, Dong-an Wang, H. Harry Asada, and Peter C. Y. Chen. “Three-Dimensional Characterization of Mechanical Interactions Between Endothelial Cells and Extracellular Matrix During Angiogenic Sprouting.” Scientific Reports 6 (February 23, 2016): 21362.
Version
Final published version
Abstract
We studied the three-dimensional cell-extracellular matrix interactions of endothelial cells that form multicellular structures called sprouts. We analyzed the data collected in-situ from angiogenic sprouting experiments and identified the differentiated interaction behavior exhibited by the tip and stalk cells. Moreover, our analysis of the tip cell lamellipodia revealed the diversity in their interaction behavior under certain conditions (e.g., when the heading of a sprout is switched approximately between the long-axis direction of two different lamellipodia). This study marks the first time that new characteristics of such interactions have been identified with shape changes in the sprouts and the associated rearrangements of collagen fibers. Clear illustrations of such changes are depicted in three-dimensional views.
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
Terms of Use
Creative Commons Attribution
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1038/srep21362
