Multi-color reflectance imaging of middle ear pathology in vivo
Name
216_2015_Article_8580.pdf
Size
2.08 MB
Format
Adobe PDF
Checksum (MD5)
0024ec2df987eb655a8997ecd9d4473c
Author(s)
Valdez, Tulio A
Spegazzini, Nicolas
Pandey, Rishikesh
Longo, Kaitlyn
Grindle, Christopher
Peterson, Donald
Barman, Ishan
Valdez, Tulio A.
Date Issued
March 2015
Journal
Analytical and Bioanalytical Chemistry
Publisher
Springer Berlin Heidelberg
Citation
Valdez, Tulio A., Nicolas Spegazzini, Rishikesh Pandey, Kaitlyn Longo, Christopher Grindle, Donald Peterson, and Ishan Barman. "Multi-color reflectance imaging of middle ear pathology in vivo." Analytical and Bioanalytical Chemistry 407:12 (May 2015), pp. 3277-3283.
Version
Author's final manuscript
Abstract
Otoscopic examination using white-light illumination has remained virtually unchanged for well over a century. However, the limited contrast of white-light otoscopy constrains the ability to make accurate assessment of middle ear pathology and is subject to significant observer variability. Here, we employ a modified otoscope with multi-color imaging capabilities for superior characterization of the middle ear constituents in vivo and for enhanced diagnosis of acute otitis media and cholesteatoma. In this pilot study, five patients undergoing surgery for tympanostomy tube placement and congenital cholesteatoma excision were imaged using the custom-designed multi-color video-rate reflectance imaging system. We show that the multi-color imaging approach offers an increase in image contrast, thereby enabling clear visualization of the middle ear constituents, especially of the tympanic membrane vascularity. Differential absorption at the multiple wavelengths provides a measure of biochemical and morphological information, and the rapid acquisition and analysis of these images aids in objective evaluation of the middle ear pathology. Our pilot study shows the potential of using label-free narrow-band reflectance imaging to differentiate middle ear pathological conditions from normal middle ear. This technique can aid in obtaining objective and reproducible diagnoses as well as provide assistance in guiding excisional procedures.
MIT Department
Massachusetts Institute of Technology. Department of Chemistry
Massachusetts Institute of Technology. Spectroscopy Laboratory
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1007/s00216-015-8580-y
