Talbot holographic illumination nonscanning (THIN) fluorescence microscopy
Name
nihms-657211.pdf
Size
616.14 KB
Format
Adobe PDF
Checksum (MD5)
8fb38340a146fd173d85a6bc1eeaff27
Author(s)
Luo, Yuan
Singh, Vijay Raj
Bhattacharya, Dipanjan
Tsai, Jui-Chang
Yu, Sung-Liang
Chen, Hsi-Hsun
Wong, Jau-Min
Matsudaira, Paul
So, Peter T. C.
Barbastathis, George
Date Issued
August 2014
Journal
Laser & Photonics Reviews
Publisher
Wiley
Citation
Luo, Yuan, et al. “Talbot Holographic Illumination Nonscanning (THIN) Fluorescence Microscopy: Talbot Holographic Illumination Nonscanning (THIN) Fluorescence Microscopy.” Laser & Photonics Reviews, vol. 8, no. 5, Sept. 2014, pp. L71–75.
Version
Author's final manuscript
Abstract
Optical sectioning techniques offer the ability to acquire three-dimensional information from various organ tissues by discriminating between the desired in-focus and out-of-focus (background) signals. Alternative techniques to confocal, such as active structured illumination, exist for fast optically sectioned images, but they require individual axial planes to be imaged consecutively. In this article, an imaging technique (THIN), by utilizing active Talbot illumination in 3D and multiplexed holographic Bragg filters for depth discrimination, is demonstrated for imaging in vivo 3D biopsy without mechanical or optical axial scanning. Optical sectioning techniques offer the ability to acquire three-dimensional images by discriminating the desired in-focus signal from out-of-focus (background) signals. Alternative existing techniques require scanning laterally or imaging individual axial planes consecutively. Here, an imaging technique (THIN), by utilizing the active illumination and multiplexed volume holographic collection, is demonstrated to image in vivo 3D biopsy without any scanning. © 2014 by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Mechanical Engineering
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1002/LPOR.201400053
