Fontimonas thermophila Alkane Monooxygenase (FtAlkB) Is an Alkyl Fluoride Dehalogenase
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Hendricks_JACS_2025.pdf
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Author(s)
Hendricks, Lauren
Reinhardt, Clorice R
Green, Tierani
Kunczynski, Lily
Roberts, August Jaunzarins
Miller, Naomi
Rafalin, Noga
Kulik, Heather J
Groves, John T
Austin, Rachel N
Date Issued
March 7, 2025
Journal
Journal of the American Chemical Society
Publisher
American Chemical Society
Citation
Hendricks, Lauren, Reinhardt, Clorice R, Green, Tierani, Kunczynski, Lily, Roberts, August Jaunzarins et al. 2025. "Fontimonas thermophila Alkane Monooxygenase (FtAlkB) Is an Alkyl Fluoride Dehalogenase." Journal of the American Chemical Society, 147 (11).
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Author's final manuscript
Abstract
Purified alkane monooxygenase (AlkB) from Fontimonas thermophila (FtAlkB) catalyzes the defluorination of 1-
fluorooctane, producing octanal, which is partially reduced under the reaction conditions to generate 1-octanol. This reaction occurs
preferentially at the monofluorinated methyl group, with only a minor amount of oxidation at the nonfluorinated end of the
molecule. The dehalogenation chemistry is specific to 1-fluorooctane, as neither 1-chlorooctane or 1-bromooctane are dehalogenated
to an appreciable extent. Furthermore, P. putida cells containing the structurally related AlkB (PpAlkB) along with the full set of
genes required for alkane metabolism, utilize 1-fluorooctane as their sole source of carbon with growth rates comparable to those for
cells grown with octane.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Massachusetts Institute of Technology. Department of Chemistry
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DOI of Published Version
10.1021/jacs.5c00386
