RNA Pol IV induces antagonistic parent-of-origin effects on Arabidopsis endosperm
Name
journal.pbio.3001602.pdf
Description
Published version
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1.46 MB
Format
Adobe PDF
Checksum (MD5)
b4ddaaaf69d163067aedeac596a91da2
Author(s)
Satyaki, Prasad RV
Gehring, Mary
Date Issued
April 2022
Journal
PLOS Biology
Publisher
Public Library of Science (PLoS)
Citation
Satyaki, Prasad RV and Gehring, Mary. 2022. "RNA Pol IV induces antagonistic parent-of-origin effects on Arabidopsis endosperm." PLOS Biology, 20 (4).
Version
Final published version
Abstract
Gene expression in endosperm—a seed tissue that mediates transfer of maternal resources to offspring—is under complex epigenetic control. We show here that plant-specific RNA polymerase IV (Pol IV) mediates parental control of endosperm gene expression. Pol IV is required for the production of small interfering RNAs that typically direct DNA methylation. We compared small RNAs (sRNAs), DNA methylation, and mRNAs in Arabidopsis thaliana endosperm from heterozygotes produced by reciprocally crossing wild-type (WT) plants to Pol IV mutants. We find that maternally and paternally acting Pol IV induce distinct effects on endosperm. Loss of maternal or paternal Pol IV impacts sRNAs and DNA methylation at different genomic sites. Strikingly, maternally and paternally acting Pol IV have antagonistic impacts on gene expression at some loci, divergently promoting or repressing endosperm gene expression. Antagonistic parent-of-origin effects have only rarely been described and are consistent with a gene regulatory system evolving under parental conflict.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Whitehead Institute for Biomedical Research
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Creative Commons Attribution 4.0 International License
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DOI of Published Version
https://doi.org/10.1371/journal.pbio.3001602
