Unexpected connections between type VI-B CRISPR-Cas systems, bacterial natural competence, ubiquitin signaling network and DNA modification through a distinct family of membrane proteins
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fnz088.pdf
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Published version
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1.78 MB
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Author(s)
Makarova, Kira S
Gao, Linyi
Zhang, Feng
Koonin, Eugene V
Date Issued
2019
Journal
FEMS Microbiology Letters
Publisher
Oxford University Press (OUP)
Citation
Makarova, Kira S, Gao, Linyi, Zhang, Feng and Koonin, Eugene V. 2019. "Unexpected connections between type VI-B CRISPR-Cas systems, bacterial natural competence, ubiquitin signaling network and DNA modification through a distinct family of membrane proteins." FEMS Microbiology Letters, 366 (8).
Version
Final published version
Abstract
In addition to core Cas proteins, CRISPR-Cas loci often encode ancillary proteins that modulate the activity of the respective effectors in interference. Subtype VI-B1 CRISPR-Cas systems encode the Csx27 protein that down-regulates the activity of Cas13b when the type VI-B locus is expressed in Escherichia coli. We show that Csx27 belongs to an expansive family of proteins that contain four predicted transmembrane helices and are typically encoded in predicted operons with components of the bacterial natural transformation machinery, multidomain proteins that consist of components of the ubiquitin signaling system and proteins containing the ligand-binding WYL domain and a helix-turn-helix domain. The Csx27 family proteins are predicted to form membrane channels for ssDNA that might comprise the core of a putative novel, Ub-regulated system for DNA uptake and, possibly, degradation. In addition to these associations, a distinct subfamily of the Csx27 family appears to be a part of a novel, membrane-associated system for DNA modification. In Bacteroidetes, subtype VI-B1 systems might degrade nascent transcripts of foreign DNA in conjunction with its uptake by the bacterial cell. These predictions suggest several experimental directions for the study of type VI CRISPR-Cas systems and distinct mechanisms of foreign DNA uptake and degradation in bacteria.
MIT Department
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
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This work is written by (a) US Government employee(s) and is in the public domain in the US
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DOI of Published Version
https://doi.org/10.1093/FEMSLE/FNZ088
