Bipolar Localization of the Replication Origin Regions of Chromosomes in Vegetative and Sporulating Cells of B. subtilis
Name
Grossman_Bipolar localization.pdf
Size
509.21 KB
Format
Adobe PDF
Checksum (MD5)
d91844fc2484ae3fb12d2d4c2bf42efa
Author(s)
Webb, Chris D
Teleman, Aurelio
Gordon, Scott
Straight, Aaron
Belmont, Andrew
Lin, Daniel Chi-Hong
Wright, Andrew
Losick, Richard
Grossman, Alan Davis
Date Issued
March 1997
Journal
Cell
Publisher
Elsevier
Citation
Webb, Chris D, Aurelio Teleman, Scott Gordon, Aaron Straight, Andrew Belmont, Daniel Chi-Hong Lin, Alan D Grossman, Andrew Wright, and Richard Losick. “Bipolar Localization of the Replication Origin Regions of Chromosomes in Vegetative and Sporulating Cells of B. subtilis.” Cell 88, no. 5 (March 1997): 667-674. Copyright © 1997 Cell Press
Version
Final published version
Abstract
To investigate chromosome segregation in B. subtilis, we introduced tandem copies of the lactose operon operator into the chromosome near the replication origin or terminus. We then visualized the position of the operator cassettes with green fluorescent protein fused to the LacI repressor. In sporulating bacteria, which undergo asymmetric cell division, origins localized near each pole of the cell whereas termini were restricted to the middle. In growing cells, which undergo binary fission, origins were observed at various positions but preferentially toward the poles early in the cell cycle. In contrast, termini showed little preference for the poles. These results indicate the existence of a mitotic-like apparatus that is responsible for moving the origin regions of newly formed chromosomes toward opposite ends of the cell.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1016/S0092-8674(00)81909-1
