Invertible promoters mediate bacterial phase variation, antibiotic resistance, and host adaptation in the gut
Name
nihms-1022970.pdf
Description
Accepted version
Size
976.46 KB
Format
Adobe PDF
Checksum (MD5)
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Author(s)
Jiang, Xiaofang
Hall, A. Brantley
Arthur, Timothy D.
Plitchta, Damian R.
Covington, Christian T.
Poyet, Mathilde
Crothers, Jessica
Moses, Peter L.
Tolonen, Andrew C.
Vlamakis, Hera
Date Issued
January 2019
Journal
Methods
Publisher
American Association for the Advancement of Science (AAAS)
Citation
Hosseini, Poorya, et al. “Single-Shot Dual-Wavelength Interferometric Microscopy.” Methods 136 (March 2018): 35–39.
Version
Author's final manuscript
Abstract
Phase variation, the reversible alternation between genetic states, enables infection by pathogens and colonization by commensals. However, the diversity of phase variation remains underexplored. We developed the PhaseFinder algorithm to quantify DNA inversion–mediated phase variation. A systematic search of 54,875 bacterial genomes identified 4686 intergenic invertible DNA regions (invertons), revealing an enrichment in host-associated bacteria. Invertons containing promoters often regulate extracellular products, underscoring the importance of surface diversity for gut colonization. We found invertons containing promoters regulating antibiotic resistance genes that shift to the ON orientation after antibiotic treatment in human metagenomic data and in vitro, thereby mitigating the cost of antibiotic resistance. We observed that the orientations of some invertons diverge after fecal microbiota transplant, potentially as a result of individual-specific selective forces.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1126/SCIENCE.AAU5238
