Multidimensional chemical control of CRISPR–Cas9
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nihms876240.pdf
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Author(s)
Maji, Basudeb
Shoulders, Matthew D
Choudhary, Amit
Moore, Christopher
Zetsche, Bernd
Volz, Sara E.
Zhang, Feng
Date Issued
October 2016
Journal
Nature Chemical Biology
Publisher
Nature Publishing Group
Citation
Maji, Basudeb et al. "Multidimensional chemical control of CRISPR–Cas9." Nature Chemical Biology 13 (January 2017): 9-11 © 2016 The Author(s)
Version
Author's final manuscript
Abstract
Cas9-based technologies have transformed genome engineering and the interrogation of genomic functions, but methods to control such technologies across numerous dimensions-including dose, time, specificity, and mutually exclusive modulation of multiple genes-are still lacking. We conferred such multidimensional controls to diverse Cas9 systems by leveraging small-molecule-regulated protein degron domains. Application of our strategy to both Cas9-mediated genome editing and transcriptional activities opens new avenues for systematic genome interrogation.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Massachusetts Institute of Technology. Department of Chemistry
McGovern Institute for Brain Research at MIT
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Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1038/NCHEMBIO.2224
