Optical diffraction tomography for high resolution live cell imaging
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Author(s)
Sung, Yongjin
Choi, Wonshik
Fang-Yen, Chris
Badizadegan, Kamran
Dasari, Ramachandra Rao
Feld, Michael S.
Date Issued
January 2009
Journal
Optics Express
Publisher
Optical Society of America
Citation
Yongjin Sung, Wonshik Choi, Christopher Fang-Yen, Kamran Badizadegan, Ramachandra R. Dasari, and Michael S. Feld, "Optical diffraction tomography for high resolution live cell imaging," Opt. Express 17, 266-277 (2009)
http://www.opticsinfobase.org/abstract.cfm?URI=oe-17-1-266
Version
Author's final manuscript
Abstract
We report the experimental implementation of optical diffraction
tomography for quantitative 3D mapping of refractive index in live
biological cells. Using a heterodyne Mach-Zehnder interferometer, we
record complex field images of light transmitted through a sample with
varying directions of illumination. To quantitatively reconstruct the 3D map
of complex refractive index in live cells, we apply optical diffraction
tomography based on the Rytov approximation. In this way, the effect of
diffraction is taken into account in the reconstruction process and
diffraction-free high resolution 3D images are obtained throughout the
entire sample volume. The quantitative refractive index map can potentially
serve as an intrinsic assay to provide the molecular concentrations without
the addition of exogenous agents and also to provide a method for studying
the light scattering properties of single cells.
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
Massachusetts Institute of Technology. Department of Physics
Massachusetts Institute of Technology. Spectroscopy Laboratory
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DOI of Published Version
http://dx.doi.org/10.1364/OE.17.000266
