Implantable Nanosensors for Human Steroid Hormone Sensing In Vivo Using a Self‐Templating Corona Phase Molecular Recognition
Name
10.1002-adhm.202000429.pdf
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2.03 MB
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Checksum (MD5)
a1c0e99b1f3aa6b96b224628471eacb0
Author(s)
Lee, Michael A.
Wang, Song
Jin, Xiaojia
Bakh, Naveed Ali
Nguyen, Freddy T.
Dong, Juyao
Silmore, Kevin S.
Gong, Xun
Pham, Crystal
Jones, Kelvin K.
Date Issued
September 16, 2020
Journal
Advanced Healthcare Materials
Publisher
Wiley
Citation
Lee, M. A., Wang, S., Jin, X., Bakh, N. A., Nguyen, F. T., Dong, J., Silmore, K. S., Gong, X., Pham, C., Jones, K. K., Muthupalani, S., Bisker, G., Son, M., Strano, M. S., Implantable Nanosensors for Human Steroid Hormone Sensing In Vivo Using a Self-Templating Corona Phase Molecular Recognition. Adv. Healthcare Mater. 2020, 9, 2000429.
Version
Author's final manuscript
Abstract
Dynamic measurements of steroid hormones in vivo are critical, but steroid sensing is currently limited by the availability of specific molecular recognition elements due to the chemical similarity of these hormones. In this work, a new, self-templating synthetic approach is applied using corona phase molecular recognition (CoPhMoRe) targeting the steroid family of molecules to produce near infrared fluorescent, implantable sensors. A key limitation of CoPhMoRe has been its reliance on library generation for sensor screening. This problem is addressed with a self-templating strategy of polymer design, using the examples of progesterone and cortisol sensing based on a styrene and acrylic acid copolymer library augmented with an acrylated steroid. The pendant steroid attached to the corona backbone is shown to self-template the phase, providing a unique CoPhMoRE design strategy with high efficacy. The resulting sensors exhibit excellent stability and reversibility upon repeated analyte cycling. It is shown that molecular recognition using such constructs is viable even in vivo after sensor implantation into a murine model by employing a poly (ethylene glycol) diacrylate (PEGDA) hydrogel and porous cellulose interface to limit nonspecific absorption. The results demonstrate that CoPhMoRe templating is sufficiently robust to enable a new class of continuous, in vivo biosensors.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Massachusetts Institute of Technology. Division of Comparative Medicine
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DOI of Published Version
https://doi.org/10.1002/adhm.202000429
