Tools for live-cell imaging of cytoskeletal and nuclear behavior in the unconventional yeast, Aureobasidium pullulans
Name
tools-for-live-cell-imaging-of-cytoskeletal-and-nuclear-behavior-in-the-unconventional-yeast-aureobasidium-pullulans.pdf
Description
Published version
Size
2.82 MB
Format
Adobe PDF
Checksum (MD5)
53a5bb30976bc2246082415ecf35e094
Author(s)
Petrucco, Claudia A
Crocker, Alex W
D’Alessandro, Alec
Medina, Edgar M
Gorman, Olivia
McNeill, Jessica
Gladfelter, Amy S
Lew, Daniel J
Date Issued
April 1, 2024
Journal
Molecular Biology of the Cell
Publisher
American Society for Cell Biology (ASCB)
Citation
Petrucco, Claudia A, Crocker, Alex W, D’Alessandro, Alec, Medina, Edgar M, Gorman, Olivia et al. 2024. "Tools for live-cell imaging of cytoskeletal and nuclear behavior in the unconventional yeast, Aureobasidium pullulans." Molecular Biology of the Cell, 35 (4).
Version
Final published version
Abstract
Aureobasidium pullulans is a ubiquitous fungus with a wide variety of morphologies and growth modes including “typical” single-budding yeast, and interestingly, larger multinucleate yeast than can make multiple buds in a single cell cycle. The study of A. pullulans promises to uncover novel cell biology, but currently tools are lacking to achieve this goal. Here, we describe initial components of a cell biology toolkit for A. pullulans, which is used to express and image fluorescent probes for nuclei as well as components of the cytoskeleton. These tools allowed live-cell imaging of the multinucleate and multibudding cycles, revealing highly synchronous mitoses in multinucleate yeast that occur in a semiopen manner with an intact but permeable nuclear envelope. These findings open the door to using this ubiquitous polyextremotolerant fungus as a model for evolutionary cell biology.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Creative Commons Attribution-Noncommercial-ShareAlike
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1091/mbc.E23-10-0388
