Asymmetric apportioning of aged mitochondria between daughter cells is required for stemness
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Sabatini_Asymmetric apportioning.pdf
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Author(s)
Chen, Walter W.
Marjanovic, Nemanja
Zoncu, Roberto
Katajisto, Pekka
Dohla, Julia
Chaffer, Christine L.
Pentinmikko, Nalle
Iqbal, Sharif
Weinberg, Robert A
Sabatini, David
Date Issued
April 2015
Journal
Science
Publisher
American Association for the Advancement of Science (AAAS)
Citation
Katajisto, Pekka, Julia Dohla, Christine L. Chaffer, Nalle Pentinmikko, Nemanja Marjanovic, Sharif Iqbal, Roberto Zoncu, Walter Chen, Robert A. Weinberg, and David M. Sabatini. “Asymmetric Apportioning of Aged Mitochondria Between Daughter Cells Is Required for Stemness.” Science 348, no. 6232 (April 2, 2015): 340–343.
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Author's final manuscript
Abstract
By dividing asymmetrically, stem cells can generate two daughter cells with distinct fates. However, evidence is limited in mammalian systems for the selective apportioning of subcellular contents between daughters. We followed the fates of old and young organelles during the division of human mammary stemlike cells and found that such cells apportion aged mitochondria asymmetrically between daughter cells. Daughter cells that received fewer old mitochondria maintained stem cell traits. Inhibition of mitochondrial fission disrupted both the age-dependent subcellular localization and segregation of mitochondria and caused loss of stem cell properties in the progeny cells. Hence, mechanisms exist for mammalian stemlike cells to asymmetrically sort aged and young mitochondria, and these are important for maintaining stemness properties.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Whitehead Institute for Biomedical Research
Koch Institute for Integrative Cancer Research at MIT
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1126/science.1260384
