Genetic networks controlled by the bacterial replication initiator and transcription factor DnaA in Bacillus subtilis
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nihms897792.pdf
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Accepted version
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1.4 MB
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Author(s)
Washington, Tracy A.
Smith, Janet L.
Grossman, Alan Davis
Alternative Title
Genes controlled by DnaA
Date Issued
August 2017
Journal
Molecular Microbiology
Publisher
Wiley
Citation
Washington, Tracy A. et al. "Genetic networks controlled by the bacterial replication initiator and transcription factor DnaA in Bacillus subtilis." Molecular Microbiology 106, 1 (August 2017): 109-128 © 2017 John Wiley & Sons Ltd
Version
Author's final manuscript
Abstract
DnaA is the widely conserved bacterial AAA+ ATPase that functions as both the replication initiator and a transcription factor. In many organisms, DnaA controls expression of its own gene and likely several others during growth and in response to replication stress. To evaluate the effects of DnaA on gene expression, separate from its role in replication initiation, we analyzed changes in mRNA levels in Bacillus subtilis cells with and without dnaA, using engineered strains in which dnaA is not essential. We found that dnaA was required for many of the changes in gene expression in response to replication stress. We also found that dnaA indirectly affected expression of several regulons during growth, including those controlled by the transcription factors Spo0A, AbrB, PhoP, SinR, RemA, Rok and YvrH. These effects were largely mediated by the effects of DnaA on expression of sda. DnaA activates transcription of sda, and Sda inhibits histidine protein kinases required for activation of the transcription factor Spo0A. We also found that loss of dnaA caused a decrease in the development of genetic competence. Together, our results indicate that DnaA plays an important role in modulating cell physiology, separate from its role in replication initiation.
MIT Department
Massachusetts Institute of Technology. Department of Biology
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DOI of Published Version
https://doi.org/10.1111/mmi.13755
