Fluorescence Sheds Light on DNA Damage, DNA Repair, and Mutations
Name
nihms-1651073.pdf
Description
Accepted version
Size
515.51 KB
Format
Unknown
Checksum (MD5)
75b141f6373ab6fb19d2a3231cf51d88
Author(s)
Owiti, Norah Auma
Nagel, Zachary D
Engelward, Bevin P
Date Issued
March 2021
Journal
Trends in Cancer
Publisher
Elsevier BV
Citation
Owiti, Norah A, Nagel, Zachary D and Engelward, Bevin P. 2021. "Fluorescence Sheds Light on DNA Damage, DNA Repair, and Mutations." Trends in Cancer, 7 (3).
Version
Author's final manuscript
Abstract
© 2020 Elsevier Inc. DNA damage can lead to carcinogenic mutations and toxicity that promotes diseases. Therefore, having rapid assays to quantify DNA damage, DNA repair, mutations, and cytotoxicity is broadly relevant to health. For example, DNA damage assays can be used to screen chemicals for genotoxicity, and knowledge about DNA repair capacity has applications in precision prevention and in personalized medicine. Furthermore, knowledge of mutation frequency has predictive power for downstream cancer, and assays for cytotoxicity can predict deleterious health effects. Tests for all of these purposes have been rendered faster and more effective via adoption of fluorescent readouts. Here, we provide an overview of established and emerging cell-based assays that exploit fluorescence for studies of DNA damage and its consequences.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1016/J.TRECAN.2020.10.006
