Enhanced electrohydrodynamic collapse of DNA due to dilute polymers
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Doyle_Enhanced.pdf
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Author(s)
Du, Ning
Doyle, Patrick S.
Renner, C. Benjamin
Date Issued
May 2014
Journal
Biomicrofluidics
Publisher
American Institute of Physics (AIP)
Citation
Benjamin Renner, C., Ning Du, and Patrick S. Doyle. “Enhanced Electrohydrodynamic Collapse of DNA Due to Dilute Polymers.” Biomicrofluidics 8, no. 3 (May 2014): 034103. © 2014 AIP Publishing LLC
Version
Final published version
Abstract
We experimentally demonstrate that addition of small, charge-neutral polymers to a buffer solution can promote compression of dilute solutions of single electrophoresing DNA. This phenomenon contrasts with the observed extension of DNA during capillary electrophoresis in dilute solutions of high molecular weight polymers. We propose these discrepancies in micron-scale DNA configurations arise from different nano-scale DNA-polymer collision events, controlled by solute polymer properties. We build upon theories previously proposed for intermolecular DNA aggregation in polymer-free solutions to develop scaling theories that describe trends seen in our data for intramolecular DNA compaction in dilute polymer solutions.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1063/1.4878135
