Enhanced Axial Resolution of Wide-Field Two-Photon Excitation Microscopy by Line Scanning Using a Digital Micromirror Device
Name
Enhanced axial.pdf
Size
1.9 MB
Format
Adobe PDF
Checksum (MD5)
49f89e531effc56a3d39348da2a2bb7b
Author(s)
Rowlands, Christopher
Park, Jong Kang
So, Peter T. C.
Date Issued
March 2017
Journal
Micromachines
Publisher
MDPI AG
Citation
Park, Jong; Rowlands, Christopher and So, Peter. “Enhanced Axial Resolution of Wide-Field Two-Photon Excitation Microscopy by Line Scanning Using a Digital Micromirror Device.” Micromachines 8, no. 3 (March 2017): 85 © 2017 The Author(s)
Version
Final published version
Abstract
Temporal focusing multiphoton microscopy is a technique for performing highly parallelized multiphoton microscopy while still maintaining depth discrimination. While the conventional wide-field configuration for temporal focusing suffers from sub-optimal axial resolution, line scanning temporal focusing, implemented here using a digital micromirror device (DMD), can provide substantial improvement. The DMD-based line scanning temporal focusing technique dynamically trades off the degree of parallelization, and hence imaging speed, for axial resolution, allowing performance parameters to be adapted to the experimental requirements. We demonstrate this new instrument in calibration specimens and in biological specimens, including a mouse kidney slice.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Laser Biomedical Research Center
Terms of Use
Creative Commons Attribution 4.0 International License
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.3390/mi8030085
