The Outgrowth of Micrometastases Is Enabled by the Formation of Filopodium-like Protrusions
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Author(s)
Shibue, Tsukasa
Brooks, Mary W.
Inan, M. Fatih
Reinhardt, Ferenc
Weinberg, Robert A
Date Issued
May 2012
Journal
Cancer Discovery
Publisher
American Association for Cancer Research (AACR)
Citation
Shibue, Tsukasa et al. “The Outgrowth of Micrometastases Is Enabled by the Formation of Filopodium-Like Protrusions.” Cancer Discovery 2, 8 (May 2012): 706–721 © 2012 American Association for Cancer Research
Version
Author's final manuscript
Abstract
Disseminated cancer cells that have extravasated into the tissue parenchyma must interact productively with its extracellular matrix components to survive, proliferate, and form macroscopic metastases. The biochemical and cell biologic mechanisms enabling this interaction remain poorly understood. We find that the formation of elongated integrin β1-containing adhesion plaques by cancer cells that have extravasated into the lung parenchyma enables the proliferation of these cells via activation of focal adhesion kinase. These plaques originate in and appear only after the formation of filopodium-like protrusions (FLP) that harbor integrin β1 along their shafts. The cytoskeleton-regulating proteins Rif and mDia2 contribute critically to the formation of these protrusions and thereby enable the proliferation of extravasated cancer cells. Hence, the formation of FLPs represents a critical rate-limiting step for the subsequent development of macroscopic metastases. SIGNIFICANCE: Although the mechanisms of metastatic dissemination have begun to be uncovered, those involved in the establishment of extravasated cancer cells in foreign tissue microenvironments remained largely obscure. We have studied the behavior of recently extravasated cancer cells in the lungs and identified a series of cell biologic processes involving the formation of filopodium-like protrusions and the subsequent development of elongated, mature adhesion plaques, which contribute critically to the rapid proliferation of the micrometastatic cells and thus are prerequisites to the eventual lung colonization by these cells.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Massachusetts Institute of Technology. Division of Comparative Medicine
Ludwig Center for Molecular Oncology (Massachusetts Institute of Technology)
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DOI of Published Version
https://doi.org/10.1158/2159-8290.CD-11-0239
