Characterization of a perfused 3D liver bioreactor
Name
191731091-MIT.pdf
Description
Full printable version
Size
1.69 MB
Format
Adobe PDF
Checksum (MD5)
c636edbed90084f39b30bbdcc5fdbc11
Author(s)
Mitchel, Jennifer (Jennifer A.)
Advisor(s)
Linda G. Griffith.
Alternative Title
Characterization of a perfused three dimension liver bioreactor
Date Issued
2007
Publisher
Massachusetts Institute of Technology
Abstract
The liver is the most important site of drug and nutrient metabolism in the body, and we desire an accurate in vitro model that allows us to perform long term drug and metabolism studies. To this end of developing an assaying tool, I used an existing multi-well bioreactor that allows for formation of perfused, three dimensional tissue structures, and began characterization of tissue behavior over time. One issue in the multi-well bioreactor is the unknown profile of cell retention over time, which is an important specification for normalizing data from drug metabolism studies. Number of cells can be indirectly assessed by measuring total protein or RNA levels when direct counting is problematic. To the end of comparing these methods, an additional goal of this thesis was to develop a protocol to measure both protein and RNA levels from a single sample using the commercially available reagent RNAlater. RNAlater was shown, however, to be incompatible with certain existing protocols for isolating both protein and RNA.
Description
Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2007.
Includes bibliographical references (leaves 17-18).
Subjects
Mechanical Engineering.
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
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