Portable iso-dielectrophoresis system
Name
965664704-MIT.pdf
Description
Full printable version
Size
2.94 MB
Format
Adobe PDF
Checksum (MD5)
b2272b19e669f8a57d9c18c6e5e7c6e3
Author(s)
Liu, Lisa, M. Eng. Massachusetts Institute of Technology
Advisor(s)
Joel Voldman.
Alternative Title
Portable IDS system
Date Issued
2016
Publisher
Massachusetts Institute of Technology
Abstract
Iso-dielectrophoresis (IDS) can be used to characterize and separate cells by their electrical characteristics. An IDS system usually consist of a RF frequency voltage source up to 24 V, syringe pumps, microscope, and computer. Much of this equipment is difficult to transport, making off-site sample collection of time-sensitive samples, such as blood, difficult. The miniaturization of the IDS system would allow measurements to take place at the time of sample collection, improving the confidence in the measurement. This thesis details an attempt to miniaturize each system. The Raspberry Pi microcomputer was used to control and process signals from the hardware. A portable, affordable system for the electrical and optical equipment systems are described. A custom PCB was designed to provide voltage signals up to 3 MHz and 12 Vpp. A microscope using a Raspberry Pi camera and reversed webcam lens achieved 240x magnification with 13 [mu]m resolution. Alternatives to the syringe pump, including air pressure regulated pumps and peristaltic pumps, were considered for the fluidic component.
Description
Thesis: M. Eng., Massachusetts Institute of Technology, Department of Electrical Engineering and Computer Science, 2016.
This electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections.
Cataloged from student-submitted PDF version of thesis.
Includes bibliographical references (pages 60-62).
Subjects
Electrical Engineering and Computer Science.
MIT Department
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Terms of Use
M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.
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