Conformation and dynamics of the mammalian chromosome
Name
952599982-MIT.pdf
Description
Full printable version
Size
7.92 MB
Format
Adobe PDF
Checksum (MD5)
9c4662dcbb80b4849c93c83a874a8d52
Author(s)
Zhang, Lyndon N. (Lyndon Nuoxi)
Advisor(s)
Ibrahim I. Cissé.
Date Issued
2016
Publisher
Massachusetts Institute of Technology
Abstract
The control of transcription represents a fundamental, initial mechanism by which the regulation of gene expression is implemented. However, while much research has been done on the biochemistry and cellular function of transcription, comparatively little is known on the dynamics of transcriptional mechanisms, their impact on chromatin structure, and concomitant functional consequences. Employing chromatin immunoprecipitation measurements, we report progress towards this goal. We characterize the ensemble chromosome conformation in mouse embryonic stem cells, by measuring interaction, or contact, probabilities between distal genomic loci. We map and describe chromosome loops, consisting of two interacting CTCF sites co-bound by cohesin, that maintain the expression of genes known to promote cell identity, and restrict the expression of genes specifying repressed developmental lineages.
Description
Thesis: S.M., Massachusetts Institute of Technology, Department of Biology, 2016.
This electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections.
Cataloged from student-submitted PDF version of thesis.
Includes bibliographical references (pages 29-30).
Subjects
Biology.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Terms of Use
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