Modeling two-photon calcium fluorescence of episodic V1 recordings using multifrequency analysis
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Author(s)
Zheng, H. W.
Malik, Wasim Qamar
Runyan, Caroline Anne
Sur, Mriganka
Brown, Emery Neal
Date Issued
December 2011
Journal
2011 Annual International Conference of the IEEE Engineering in Medicine and Biology Society
Publisher
Institute of Electrical and Electronics Engineers (IEEE)
Citation
Zheng, H. W. et al. “Modeling Two-Photon Calcium Fluorescence of Episodic V1 Recordings Using Multifrequency Analysis.” 2011 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, August 30 - September 3 2011, Boston, Massachusetts, USA, Institute of Electrical and Electronics Engineers (IEEE), December 2011 © 2011 Institute of Electrical and Electronics Engineers (IEEE)
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Author's final manuscript
Abstract
The use of two-photon microscopy allows for imaging of deep neural tissue in vivo. This paper examines frequency-based analysis to two-photon calcium fluorescence images with the goal of deriving smooth tuning curves. We present a multifrequency analysis approach for improved extraction of calcium responses in episodic stimulation experiments, that is, when the stimulus is applied for a number of frames, then turned off for the next few frames, and so on. Episodic orientation stimulus was applied while recording from the primary visual cortex of an anesthetized mouse. The multifrequency model demonstrated improved tuning curve descriptions of the neurons. It also offers perspective regarding the characteristics of calcium fluorescence imaging of the brain.
MIT Department
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Picower Institute for Learning and Memory
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Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1109/IEMBS.2011.6090826
