Parabrachial Nucleus Activity in Nociception and Pain in Awake Mice
Name
5656.full.pdf
Description
Published version
Size
2.65 MB
Format
Adobe PDF
Checksum (MD5)
b537f86dd7b7661d7b56d31395f099de
Author(s)
Smith, Jesse A
Ji, Yadong
Lorsung, Rebecca
Breault, Macauley S
Koenig, Jeffrey
Cramer, Nathan
Masri, Radi
Keller, Asaf
Date Issued
August 2, 2023
Journal
The Journal of Neuroscience
Publisher
Society for Neuroscience
Citation
Jesse A. Smith, Yadong Ji, Rebecca Lorsung, Macauley S. Breault, Jeffrey Koenig, Nathan Cramer, Radi Masri, Asaf Keller, Journal of Neuroscience 2 August 2023, 43 (31) 5656-5667.
Version
Final published version
Abstract
The parabrachial nuclear complex (PBN) is a nexus for aversion and for the sensory and affective components of pain perception. We have previously shown that during chronic pain PBN neurons in anesthetized rodents have amplified activity. We report a method to record from PBN neurons of behaving, head-restrained mice while applying reproducible noxious stimuli. We find that both spontaneous and evoked activity are higher in awake animals compared with urethane anesthetized mice. Fiber photometry of calcium responses from calcitonin-gene-related peptide–expressing PBN neurons demonstrates that these neurons respond to noxious stimuli. In both males and females with neuropathic or inflammatory pain, responses of PBN neurons remain amplified for at least 5 weeks, in parallel with increased pain metrics. We also show that PBN neurons can be rapidly conditioned to respond to innocuous stimuli after pairing with noxious stimuli. Finally, we demonstrate that changes in PBN neuronal activity are correlated with changes in arousal, measured as changes in pupil area.
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DOI of Published Version
https://doi.org/10.1523/JNEUROSCI.0587-23.2023
