Tracing of Human Tumor Cell Lineages by Mitochondrial Mutations
Name
fonc-10-523860.pdf
Description
Published version
Size
2.14 MB
Format
Unknown
Checksum (MD5)
9ebd29be697a38964d0fa67909c40bdd
Author(s)
Refinetti, Paulo
Morgenthaler, Stephan
Thilly, William G
Arstad, Christian
Ekstrøm, Per O
Date Issued
2020
Journal
Frontiers in Oncology
Publisher
Frontiers Media SA
Version
Final published version
Abstract
© Copyright © 2020 Refinetti, Morgenthaler, Thilly, Arstad and Ekstrøm. Background: Previous studies have shown the value in studying lineage tracing in slices of human tumors. However, a tumor is not a two-dimensional structure and to better understand how a tumor, and its corresponding metastasis grow, a three-dimensional (3-D) view is necessary. Results: Using somatic mitochondrial mutations as a marker for lineage tracing, it is possible to identify and follow tumor specific cell lineages. Using cycling temperature capillary electrophoresis (CTCE) a total of 8 tissues from 5 patients (4 primary tumors and 4 metastasis) containing clear mitochondrial markers of tumor lineages were selected. From these 8 tissues over 9,500 laser capture microdisection (LCM) samples were taken and analyzed, in a way that allows 3-D rendering of the observations. Conclusion: Using CTCE combined with LCM makes it possible to study the 3-D patterns formed by tumors and metastasis as they grow. These results clearly show that the majority of the volume occupied by a tumor is not composed of tumor derived cells. These cells are most likely recruited from the neighboring tissue.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Terms of Use
Creative Commons Attribution 4.0 International license
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.3389/FONC.2020.523860
