CEL-Seq2: sensitive highly-multiplexed single-cell RNA-Seq
Name
Regev_CEL-Seq2.pdf
Size
2.42 MB
Format
Adobe PDF
Checksum (MD5)
5e227e379278a6eb1a7fecaf7ca41041
Author(s)
Hashimshony, Tamar
Senderovich, Naftalie
Avital, Gal
Klochendler, Agnes
de Leeuw, Yaron
Anavy, Leon
Li, Shuqiang
Livak, Kenneth J.
Dor, Yuval
Yanai, Itai
Date Issued
April 2016
Journal
Genome Biology
Publisher
Biomed Central Ltd.
Citation
Hashimshony, Tamar et al. “CEL-Seq2: Sensitive Highly-Multiplexed Single-Cell RNA-Seq.” Genome Biology 17.1 (2016): n. pag.
Version
Final published version
Abstract
Single-cell transcriptomics requires a method that is sensitive, accurate, and reproducible. Here, we present CEL-Seq2, a modified version of our CEL-Seq method, with threefold higher sensitivity, lower costs, and less hands-on time. We implemented CEL-Seq2 on Fluidigm’s C1 system, providing its first single-cell, on-chip barcoding method, and we detected gene expression changes accompanying the progression through the cell cycle in mouse fibroblast cells. We also compare with Smart-Seq to demonstrate CEL-Seq2’s increased sensitivity relative to other available methods. Collectively, the improvements make CEL-Seq2 uniquely suited to single-cell RNA-Seq analysis in terms of economics, resolution, and ease of use.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Creative Commons Attribution 4.0 International License
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1186/s13059-016-0938-8
