Design, Fabrication and Functional Analysis of a New Protein Array Based on ssDNA-based Assembly
Name
MEBCS006.pdf
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128.11 KB
Format
Adobe PDF
Checksum (MD5)
bbc40e8da449827255768048287c4d21
Author(s)
Ajikumar, Parayil Kumaran
Ng, Jin Kiat
Tang, Yew Chung
Lee, Jim Yang
Stephanopoulos, Gregory
Too, Heng-Phon
Date Issued
January 2006
Series/Report no.
Molecular Engineering of Biological and Chemical Systems (MEBCS)
Abstract
In the post genomic era, proteomics has enormous potential in biology and medicine. Among the various bioanalytical tools developed, protein microarray is one of the recent advancements which offer high throughput profiling of cellular proteins to provide insights into the mechanisms of biological processes. Fundamentally, the protein microarray involves the immobilization of interacting elements, proteins, on a few square microns of a solid support and in principle, it is capable of detecting analytes with a higher sensitivity than conventional macroscopic immunoassays. Here in the present report we delineates the design, fabrication and functional analysis of protein microarray using semi-synthetic ssDNA tagged-proteins as capturing moiety as well as address on a solid support. Optimization of the platform has been carried out by investigating various parameters such as surface chemistry, signal amplification, and conditions for homogenous liguid phase protein-protein interaction.
Subjects
Proteomics
Protein micro array
Dendrimer
glass slide
ssDNA-antibody
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