A Method for Selective Depletion of Zn(II) Ions from Complex Biological Media and Evaluation of Cellular Consequences of Zn(II) Deficiency
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nihms943608.pdf
Description
Accepted version
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531.67 KB
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Author(s)
Richardson, Christopher E. R.
Cunden, Lisa Stephanie
Butty, Vincent L G
Nolan, Elizabeth Marie
Lippard, Stephen J.
Shoulders, Matthew D.
Date Issued
January 2018
Journal
Journal of the American Chemical Society
Publisher
American Chemical Society (ACS)
Citation
Richardson, Christopher E. R. et al. "A Method for Selective Depletion of Zn(II) Ions from Complex Biological Media and Evaluation of Cellular Consequences of Zn(II) Deficiency." Journal of the American Chemical Society 140, 7 (January 2018): 2413-2416 © 2018 American Chemical Society
Version
Author's final manuscript
Abstract
We describe the preparation, evaluation, and application of an S100A12 protein-conjugated solid support, hereafter the "A12-resin", that can remove 99% of Zn(II) from complex biological solutions without significantly perturbing the concentrations of other metal ions. The A12-resin can be applied to selectively deplete Zn(II) from diverse tissue culture media and from other biological fluids, including human serum. To further demonstrate the utility of this approach, we investigated metabolic, transcriptomic, and metallomic responses of HEK293 cells cultured in medium depleted of Zn(II) using S100A12. The resulting data provide insight into how cells respond to acute Zn(II) deficiency. We expect that the A12-resin will facilitate interrogation of disrupted Zn(II) homeostasis in biological settings, uncovering novel roles for Zn(II) in biology. Keywords: peptides and proteins; metals; organic polymers; cell cultureIons
MIT Department
Massachusetts Institute of Technology. Department of Chemistry
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1021/jacs.7b12897
