Air-liquid interface culture promotes maturation and allows environmental exposure of pluripotent stem cell–derived alveolar epithelium
Name
155589.2-20220325130722-covered-e0fd13ba177f913fd3156f593ead4cfd.pdf
Description
Published version
Size
12.81 MB
Format
Adobe PDF
Checksum (MD5)
0bb92266fcc9cdd2ad4631c1dee617b1
Author(s)
Abo, Kristine M
Sainz de Aja, Julio
Lindstrom-Vautrin, Jonathan
Alysandratos, Konstantinos-Dionysios
Richards, Alexsia
Garcia-de-Alba, Carolina
Huang, Jessie
Hix, Olivia T
Werder, Rhiannon B
Bullitt, Esther
Date Issued
2022
Journal
JCI Insight
Publisher
American Society for Clinical Investigation
Citation
Abo, Kristine M, Sainz de Aja, Julio, Lindstrom-Vautrin, Jonathan, Alysandratos, Konstantinos-Dionysios, Richards, Alexsia et al. 2022. "Air-liquid interface culture promotes maturation and allows environmental exposure of pluripotent stem cell–derived alveolar epithelium." JCI Insight, 7 (6).
Version
Final published version
Abstract
Type 2 alveolar epithelial cells (AT2s), facultative progenitor cells of the lung alveolus, play a vital role in the biology of the distal lung. In vitro model systems that incorporate human cells, recapitulate the biology of primary AT2s, and interface with the outside environment could serve as useful tools to elucidate functional characteristics of AT2s in homeostasis and disease. We and others recently adapted human induced pluripotent stem cell-derived AT2s (iAT2s) for air-liquid interface (ALI) culture. Here, we comprehensively characterize the effects of ALI culture on iAT2s and benchmark their transcriptional profile relative to both freshly sorted and cultured primary human fetal and adult AT2s. We find that iAT2s cultured at ALI maintain an AT2 phenotype while upregulating expression of transcripts associated with AT2 maturation. We then leverage this platform to assay the effects of exposure to clinically significant, inhaled toxicants including cigarette smoke and electronic cigarette vapor.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Creative Commons Attribution 4.0 International license
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1172/JCI.INSIGHT.155589
