Optimization of Encoded Hydrogel Particles for Nucleic Acid Quantification
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Doyle_Optimization_encoded.pdf
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Author(s)
Pregibon, Daniel C.
Doyle, Patrick S.
Date Issued
June 2009
Journal
Analytical Chemistry
Publisher
American Chemical Society
Citation
Pregibon, Daniel C., and Patrick S. Doyle. Optimization of Encoded Hydrogel Particles for Nucleic Acid Quantification. Analytical Chemistry 81, no. 12 (June 15, 2009): 4873-4881.
Version
Author's final manuscript
Abstract
The accurate quantification of nucleic acids is of utmost importance for clinical diagnostics, drug discovery, and basic science research. These applications require the concurrent measurement of multiple targets while demanding high-throughput analysis, high sensitivity, specificity between closely related targets, and a wide dynamic range. In attempt to create a technology that can simultaneously meet these demands, we recently developed a method of multiplexed analysis using encoded hydrogel particles. Here, we demonstrate tuning of hydrogel porosity with semi-interpenetrating networks of poly(ethylene glycol), develop a quantitative model to understand hybridization kinetics, and use the findings from these studies to enhance particle design for nucleic acid detection. With an optimized particle design and efficient fluorescent labeling scheme, we demonstrate subattomole sensitivity and single-nucleotide specificity for small RNA targets.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1021/ac9005292
