Single-Cell Analysis Reveals Functionally Distinct Classes within the Planarian Stem Cell Compartment
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Reddien_Single-cell.pdf
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Author(s)
van Wolfswinkel, Josien C.
Reddien, Peter
Wagner, Daniel E.
Date Issued
July 2014
Journal
Cell Stem Cell
Publisher
Elsevier
Citation
van Wolfswinkel, Josien C., Daniel E. Wagner, and Peter W. Reddien. “Single-Cell Analysis Reveals Functionally Distinct Classes within the Planarian Stem Cell Compartment.” Cell Stem Cell 15.3 (2014): 326–339.
Version
Author's final manuscript
Abstract
Planarians are flatworms capable of regenerating any missing body region. This capacity is mediated by neoblasts, a proliferative cell population that contains pluripotent stem cells. Although population-based studies have revealed many neoblast characteristics, whether functionally distinct classes exist within this population is unclear. Here, we used high-dimensional single-cell transcriptional profiling from over a thousand individual neoblasts to directly compare gene expression fingerprints during homeostasis and regeneration. We identified two prominent neoblast classes that we named ζ (zeta) and σ (sigma). Zeta-neoblasts encompass
specified cells that give rise to an abundant postmitotic lineage including epidermal cells, and are
not required for regeneration. By contrast, sigma-neoblasts proliferate in response to injury,
possess broad lineage capacity, and can give rise to zeta-neoblasts. These findings present a new
view of planarian neoblasts, in which the population is comprised of two major and functionally
distinct cellular compartments.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Whitehead Institute for Biomedical Research
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Creative Commons Attribution-NonCommercial-NoDerivs License
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DOI of Published Version
https://doi.org/10.1016/j.stem.2014.06.007
