Dendrimer-RNA nanoparticles generate protective immunity against lethal Ebola, H1N1 influenza, and
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E4133.full.pdf
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Author(s)
Chahal, Jasdave S.
Cooper, Christopher L.
McPartlan, Justine S.
Tilley, Lucas D.
Sidik, Saima M.
Lourido, Sebastian
Bavari, Sina
Ploegh, Hidde L.
Khan, Omar Fizal
Tsosie, Jonathan
Date Issued
July 2016
Journal
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences (U.S.)
Citation
Chahal, Jasdave S. et al. “Dendrimer-RNA Nanoparticles Generate Protective Immunity Against Lethal Ebola, H1N1 Influenza, andToxoplasma Gondiichallenges with a Single Dose.” Proceedings of the National Academy of Sciences 113, 29 (July 2016): E4133–E4142 © 2016 National Academy of Sciences
Version
Final published version
Abstract
Vaccines have had broad medical impact, but existing vaccine technologies and production methods are limited in their ability to respond rapidly to evolving and emerging pathogens, or sudden outbreaks. Here, we develop a rapid-response, fully synthetic, singledose, adjuvant-free dendrimer nanoparticle vaccine platform wherein antigens are encoded by encapsulated mRNA replicons. To our knowledge, this system is the first capable of generating protective immunity against a broad spectrum of lethal pathogen challenges, including H1N1 influenza, Toxoplasma gondii, and Ebola virus. The vaccine can be formed with multiple antigenexpressing replicons, and is capable of eliciting both CD8⁺ T-cell and antibody responses. The ability to generate viable, contaminant-free vaccines within days, to single or multiple antigens, may have broad utility for a range of diseases.
MIT Department
Massachusetts Institute of Technology. Institute for Medical Engineering & Science
Massachusetts Institute of Technology. Department of Biology
Massachusetts Institute of Technology. Department of Chemical Engineering
Koch Institute for Integrative Cancer Research at MIT
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1073/PNAS.1600299113
