PIP[subscript 3] Regulates Spinule Formation in Dendritic Spines during Structural Long-Term Potentiation
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Ueda-2013-PIP3 Regulates Spinu.pdf
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Author(s)
Hayashi, Yasunori
Ueda, Yoshibumi
Date Issued
July 2013
Journal
Journal of Neuroscience
Publisher
Society for Neuroscience
Citation
Ueda, Y., and Y. Hayashi. “PIP[subscript 3] Regulates Spinule Formation in Dendritic Spines During Structural Long-Term Potentiation.” Journal of Neuroscience 33, no. 27 (July 3, 2013): 11040–11047.
Version
Final published version
Abstract
Dendritic spines are small, highly motile structures on dendritic shafts that provide flexibility to neuronal networks. Spinules are small protrusions that project from spines. The number and the length of spinules increase in response to activity including theta burst stimulation and glutamate application. However, what function spinules exert and how their formation is regulated still remains unclear. Phosphatidylinositol-3,4,5-trisphosphate (PIP[subscript 3]) plays important roles in cell motility such as filopodia and lamellipodia by recruiting downstream proteins such as Akt and WAVE to the membrane, respectively. Here we reveal that PIP[subscript 3] regulates spinule formation during structural long-term potentiation (sLTP) of single spines in CA1 pyramidal neurons of hippocampal slices from rats. Since the local distribution of PIP[subscript 3] is important to exert its functions, the subcellular distribution of PIP[subscript 3] was investigated using a fluorescence lifetime-based PIP[subscript 3] probe. PIP[subscript 3] accumulates to a greater extent in spines than in dendritic shafts, which is regulated by the subcellular activity pattern of proteins that produce and degrade PIP[subscript 3]. Subspine imaging revealed that when sLTP was induced in a single spine, PIP[subscript 3] accumulates in the spinule whereas PIP[subscript 3] concentration in the spine decreased.
MIT Department
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Picower Institute for Learning and Memory
RIKEN-MIT Neuroscience Research Center
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DOI of Published Version
https://doi.org/10.1523/jneurosci.3122-12.2013
