Permitted and restricted steps of human kinetochore assembly in mitotic cell extracts
Name
mbc.e20-07-0461.pdf
Description
Published version
Size
7.65 MB
Format
Adobe PDF
Checksum (MD5)
1419926b476d9bb441124ab0241e49d4
Author(s)
Tarasovetc, Ekaterina V
Allu, Praveen Kumar
Wimbish, Robert T
DeLuca, Jennifer G
Cheeseman, Iain M
Black, Ben E
Grishchuk, Ekaterina L
Date Issued
2021
Journal
Molecular Biology of the Cell
Publisher
American Society for Cell Biology (ASCB)
Citation
Tarasovetc, Ekaterina V, Allu, Praveen Kumar, Wimbish, Robert T, DeLuca, Jennifer G, Cheeseman, Iain M et al. 2021. "Permitted and restricted steps of human kinetochore assembly in mitotic cell extracts." Molecular Biology of the Cell, 32 (13).
Version
Final published version
Abstract
This work represents the first systematic analysis of kinetochore assembly in human mitotic cell extracts. Using recombinant and native proteins, we show that only a few assembly steps are permitted, suggesting that multiple mechanisms guard against the spurious formation of microtubule-binding kinetochore particles in the cytoplasm of mitotic cells.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1091/MBC.E20-07-0461
